454 high-throughput sequencing combines the power of the polymerase chain reaction (PCR) and pyrosequencing.
De Novo sequencing
Random shotgun sequencing without the bias of cloning provides a true sampling of a novel genome or a metagenomic sample where no prior genetic information is known.
For when you want to know “what” is there. Random shotgun sequencing provides you with a random sampling of DNA present in your environmental sample
For when you want to know “who” is there, 16s or amplicon sequencing helps answer that question. Use of PCR primers with Roche adaptors allows for simultaneously amplifying a gene of interest and prepares the sample for pyrosequencing. The high coverage of amplicon sequencing allows researchers to accurately define known mutations and well as identify new ones.
Re-sequencing of Exons and Genome fragments
If your project requires high-quality nucleotide data for a previously characterized genome, 454 pyrosequencing allows you to supplement your existing databases as well as provide you with more data for contig assembly.
Ultra deep sequencing of Exons and Genome fragments
Characterization of structural variations and SNPs are more accurate with the high depth of coverage provided by the massively parallel 454 pyrosequencing. High level of coverage assures you that you are sampling rare variants.
Paired End Sequencing
Paired end sequencing reads for 3 Kb, 8 Kb and 20 Kb distances can be generated with average sequencing tag lengths greater than 140 bp. Highly repetitive genomic regions are somewhat or wholly spanned which enhances genome assembly.
Transcriptome Analysis sequencing
Pyrosequencing of complementary DNA (cDNA) eliminates the need for cloning of insert fragments and picking of E. coli colonies. Instead, the double-stranded cDNA library is sequenced directly.
We can provide custom, normalized cDNA library construction services. We prepare libraries from all types of biological samples providing the best outcome for your starting material. Contact us directly for this specialized service.
Library generation services
Our experienced staff will quickly and efficiently prepare your samples according to Roche/454 Life Sciences protocol. They are also very skilled at performing MID barcoding as well as working with difficult samples.
Complete data assembly of your genomic data using the Roche/454 Life Sciences Newbler algorithm will be performed. The folder of the Newbler assembly will be posted along with your raw data (SFF files) on our secure FTP server for you to download.